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We frequently receive requests about instructions and procedures for performing toxicity testing.  Therefore, we have produced this list.

Whole Effluent Toxicity Testing Procedural Manuals

  • EPA-821-R-02-012 Methods for Measuring Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms (5 th Edition); October 2002
     
  • EPA-821-R-02-013 Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water to Freshwater Organisms; (4 th Edition); October 2002
     
  • EPA-821-R-02-014 Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water to Marine and Estuarine Organisms; (3 rd Edition); October 2002

Sediment Toxicity Testing Manuals

  • EPA/600/R-94/024 Methods for Measuring the Toxicity and Bioaccumulation of Sediment-Associated Contaminants with Freshwater Invertebrates
     
  • EPA/600/R-94/025 Methods for Assessing the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Amphipods

Toxicity Identification and Evaluation Manuals:

  • EPA/600/2-88/070 Generalized Methodology for Conducting Industrial Toxicity Reduction Evaluations (TRE's); April 1989
     
  • EPA-600/6-91/003 Methods for Aquatic Toxicity Identification Evaluations: Phase I Toxicity Characterization Procedures (second edition); February 1991
     
  • EPA/600/6-91/005F Toxicity Identification Evaluation: Characterization of Chronically Toxic Effluents Phase I
     
  • EPA/600/R-92/080 Methods for Aquatic Toxicity Identification Evaluations Phase II Toxicity Identification Procedures for Samples Exhibiting Acute and Chronic Toxicity; September 1993
     
  • EPA/600/R-92/081 Methods for Aquatic Toxicity Identification Evaluation Phase III Toxicity Confirmation Procedures for Samples Exhibiting Acute and Chronic Toxicity; September 1993

Other Related Documents

  • EPA 831-S-92-002 Introduction to Water Quality-Based Toxics Control for NPDES Program; March 1992
     
  • EPA/505/2-90-001 Technical Support Document for Water Quality-Based Toxicity Control; March 1991
     
  • EPA 175-B-97-001 Terms of Environment Glossary, Abbreviations and Acronyms; December 1997
     
  • EPA 821-B-00-004 Method Guidance and Recommendations for Whole Effluent Toxicity (WET) Testing (40 CFR Part 136); July 2000
     
  • EPA 833-R-00-003 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination System Program; June 2000
     
  • National Environmental Laboratory Accreditation Conference (NELAC) Standards; Standards version currently in effect: 2001 (New versions are implemented on their effective date.)
     
  • Standard Methods for the Examination of Water and Wastewater 20 th Edition 1998
     

 


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ONLINE DOCUMENT SOURCES
You can find many of these documents using the National Technical Information Service's (NTIS) product search page.  There you will be able to search for and purchase these documents.  We recommend searching for the specific EPA document number without any of the dashes and slashes (e.g. EPA600490027F instead of EPA/600/4-90/027F).  Have fun!

NTIS Product Search Page

US EPA Whole Effluent Toxicity (WET) Methods Web Site
 

Marinco Bioassay Laboratory
Marinco Bioassay Laboratory, Inc.
Marinco Bioassay Laboratory, Inc.
4569 Samuel Street
Sarasota, FL 34233
TEL: (941) 925-3594
TOLL-FREE: (800) 889-0384
http://www.toxicitylab.com